By M. B. A. Oldstone (auth.), Michael B. A. Oldstone M.D. (eds.)
M. B. A. OLDSTONE Viruses are typically studied both simply because they reason major human, animal or plant affliction or for his or her application as fabrics to probe a uncomplicated phenomenon in biology, chemistry, genetics or molecular biology. Arenaviruses are surprisingly fascinating in that they occupy either one of those different types. Arenaviruses reason serious human illnesses recognized basically because the hemor rhagic fevers happening in South and Latin the US (Bolivia: Machupo virus and Argentina: Junin virus) and in Africa (Lassa virus). simply because such viruses produce profound incapacity and should kill the individuals they infect, they seem to be a resource of financial trouble within the nations the place they're primary. extra, they supply new difficulties for well-being care body of workers as a result of the narrowing of the realm as viewers from many nations more and more go back and forth to and from those endemic components. additionally, lymphocytic choriomeningitis virus (LCMV) can infect people world wide, even supposing the disease is mainly much less disabling than these elicited by way of different arenaviruses. but LCMV is probably going of higher drawback to non-arena-virologists and experimentalists utilizing tissue tradition or animals, i. e. , employees in molecular biology, melanoma examine, virology, immunobiology, and so on. , simply because common showing cultured cells or tissues and animals used for study could be repeatedly contaminated with LCMV with no manifesting scientific affliction or cytopathology and transmit that an infection to laboratory employees (reviewed OWSTONE and PETERS 1978). for instance, HINMAN et al.
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Extra info for Arenaviruses: Genes, Proteins, and Expression
1 PFU/cell then incubated for 24 h before adding 100 IlCi/ml of [35 S1methionine in methionine-free medium. Supernatant medium was harvested at 40 hand virus purified and analyzed by SDS-PAGE as described (BUCHMEIER et al. 1978). Evident in the autoradiogram are the major viral polypeptides NP, GP1, and GP2, as well as quantitatively minor polypeptides (P) of 200 K, 75 K, 68 K, and 14 K. In other experiments we have shown that p 200 is encoded on the viral L RNA segment and that p 68 apparently shares tryptic peptides with NP.
As GPC synthesis and consequent expression of viral glycoprotein at the plasma membrane increase, so does virus production. In turn, late in the infection when virus production wanes, GPC synthesis and surface expression of the structural glycoproteins diminish. The cellular site of GPC cleavage has not been precisely defined. In our work (BUCHMEIER and OLDSTONE 1979) we found only the mature GP1 and GP2 on the surface of infected cells and virions and suggested that cleavage occurs intracellularly; however, VAN DER ZEIJST et al.
Purified LCMV disrupted by detergents liberated an antigen which reacted with antiECFA in CF test and produced a band of identity with ECF A by immunodiffusion. Department of Immunology, Scripps Clinic and Research Foundation, 10666 North Torrey Pines Road, La Jolla, CA 92037, USA Current Topics in Microbiology and Immunology, Vol. J. S. Parekh RAWLS and BUCHMEIER (1975) arrived at similar conclusions, working with the S antigens of Pichinde virus. Antisera directed against partially purified CF antigen from cells infected by Pichinde virus were shown to react against the internal nucleocapsid protein of the virus but not with surface antigens of infected cells.
Arenaviruses: Genes, Proteins, and Expression by M. B. A. Oldstone (auth.), Michael B. A. Oldstone M.D. (eds.)